Deutsches Zentrum für integrative Biodiversitätsforschung (iDiv)
Halle-Jena-Leipzig
 

Overview of the EcoMetEoR Lab.

Facilities

LC-QToFMS 

The Bruker Impact HD provides high mass resolution and high sensitivity. Those features allow measurements of accurate masses for even trace compounds and the determination of their sum formulas. Another important feature of the QToFMS is the possibility of conducting MS/MS experiments to elucidate the structures of unknown compounds.

Technical features:

  • ESI and APCI source
  • High mass resolution
  • Multi Reaction Monitoring

Examples: 

  1. Analysis of plant defensive compounds controlling feeding and oviposition preferences in insects
  2. Analysis steroid glycosides involved in plant resistance
  3. Metabolomic profiling to study effects of root parasitism and shoot herbivory

LC-QqQMS

The Bruker EVOQ LC system can be used for quantification of known target analytes. It provides high sensitivity and robust quantification over a wide concentration range.

Technical features:

  • ESI and APCI source
  • Triple Quad Mass Spectrometer
  • Single Ion Monitoring; Multi Reaction Monitoring

Examples: 

  1. Quantification of phytohormones involved in plant development and plant growth (JA, ABA, IAA, SA, JA-ILE)
  2. Quantification of glucosinolates from shoots and roots of different Brassica species
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